The paper gives an overview of Serbias participation in various forms of regional cooperation in the Balkans. This cooperation became especially important inthe post-Dayton period. In the case of Serbia, it was pushed back by the Yugoslav war, which led to international sanctions and the 1999 military intervention by NATO.Therefore,Serbias full-fledged participation in regional organisations resumed only after the regime change of October 2000.European and Euro-Atlantic orientation constitutes today the principal direction ofSerbias foreign policy and in this connection the developing of regional cooperation in the Balkans, especially in their western part, is a prerequisite for Serbias future accession to the EO, which can become possible between 2014 and 2017.Serbia occupies a central place in the Balkan region.Situated at the crossroads of Europe and the Middle East, it has experienced the influences of both eastern and western civilisations.IRecently,regional cooperation has becomea prerequisite for Serbias EU accession as an externally determined process with rigorous conditions and increasing expectations.Therefore,this article brings an overview of the role of Belgrade role in that cooperation.